Stomatológ 2023, 33(1):30-32 | DOI: 10.69658/sto.2023.007

In vitro analysis of the biocompatibility of metallic alloys for dental use

Laura Laffranchi¹, Domenico Dalessandri¹, Raffaella Belotti¹, Luca Visconti¹, Dominika Pažur², Simona Dianišková^2,*

¹ School of Dentistry, Department of Medical and Surgical Specialties, Radiological Sciences, and Public Health, University of Brescia, Piazzale Spedali Civili 1, 25123 Brescia, Italy

² Slovak Medical University, Orthodontic Postgraduate Programme, Dental School, 83303 Bratislava, Slovakia

The study was to evaluate the biocompatibility of metal alloys used in dentistry, as subjected to different corrosion attacks according to ISO 10271:2011 and ISO 22674:2016. We prepared cultures from pulp fibroblasts isolated from fresh dental pulp of third molars. Samples of 4 metal alloys, 1 cm2 diameter each, were brought in contact with pulp fibroblasts and analysed in intervals of 24 hours for 7 days to examine the cytotoxic effects by qualitative methods. Alloys A and C did not show any alteration of the qualitative parameters. Alloys D and E showed a slight zone of inhibition from Day 2. By Day 7, alloys A and C had a slight zone of inhibition, with no suffering at the cytoplasmic or cell membrane. Alloy D caused a significant decrease of cell colonies with degeneration, lysis and cytoplasmic granules. The concentrations of ions released after the attack of corrosion were primarily Co (alloy E 379 ng/cm2), Cr (alloy E 62ng/cm2), Fe (alloy A 551ng/cm2) and Si (alloy C 526 ng/cm2). The study offers significant insights for further research of innovation. It provides substantial information about this type of products in terms of quality and market impact .

Keywords: Biocompatibility, dental alloys, corrosion

Published: June 15, 2023  Show citation